On the nRF5340 DK, there are multiple analog switches that are used to connect and disconnect signals based on different scenarios.
The USB and SW6 control the signal switches by using USB_DETECT as an input to SW6. Therefore, the interface MCU can be disconnected either by unplugging the USB cable from J2 or by toggling the nRF ONLY switch SW6.
The signal controls a set of switches (U3, U5, U6) that break the connection between the nRF5340 and the interface MCU, and control the power for the interface MCU. For more information, see Interface MCU power.
Switches U3 and U5 break the connection of the Universal Asynchronous Receiver/Transmitter (UART) lines and SWD/RESET lines. In addition, the signal controls the routing of the RESET signal depending on user preference when the interface MCU is connected/disconnected.
When a shield is connected, there are two analog switches connecting the pull-up resistors to the I2C bus lines (SDA and SCL). This function is using one ground pin on the Arduino shield to control the switch. This feature can be disabled by cutting SB33. To permanently enable pull-up resistors, short SB32.