The VDDIO external reference voltage pin is available on pin header P11 (labeled TWI). This is for external voltage input and sets the reference voltage level for the General-Purpose Input/Output (GPIO)s and the TWI.
If using external VDDIO supply on P11, disconnect the VDDIO input jumper on P18. See further details in VDDIO reference voltage.
VDDIO is shown in TWI schematic.